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Antibodies bound to the blocking peptide now not bind towards the epitope to the target protein. By evaluating the staining in the blocked antibody compared to the antibody by yourself, you can see which staining is specific.
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This guide outlines more probable optimization of cellular and immunoassay parameters to make certain the best possible final results are...
This tutorial outlines even further doable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind exclusively for the target antibody and block antibody binding. These peptide commonly is made up of the epitope acknowledged by the antibody.
Focus on Info Cyclin E belongs towards the really conserved cyclin household, whose members are characterized by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Unique cyclins show unique expression and degradation styles which contribute on the temporal coordination of each and every mitotic event. Cyclin E kinds a posh with and capabilities as being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary which is degraded as cells development through S phase.
Overexpression of this gene has become noticed in lots of tumors, which leads to chromosome instability, and so may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial function in promoting mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two additional splice variants ended up documented but comprehensive nucleotide sequence information is not really still offered.
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